Journal: Cancers

Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers

doi: 10.3390/cancers14081877

Figure Lengend Snippet: Expression of SORT1 in ovarian healthy and tumoral tissues. Immunohistochemical staining of SORT1 in tissue microarrays (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in ovarian tumors. With the immunohistochemical (IHC) scoring system used, the level of expressed SORT1 ranged from 0 to a maximum of 12. ( A ) Representative SORT1 staining by IHC in ovarian healthy, primary and metastases biopsies. Nuclei are stained blue with hematoxylin. Arrows point to ovarian healthy epithelia. Black bars represent 50 µm. ( B ) IHC scores from healthy tissues ( n = 20), benign tumors ( n = 18), malignant tumors ( n = 45) and metastases ( n = 10). ( C ) RT-qPCR was used to quantify the transcript levels of SORT1 in healthy and cancerous ovarian tissues. The SORT1 transcript levels in tumor samples were segregated according to tumor grade and then compared to the values from healthy ovarian tissues. n = 8 for healthy and for Grade I tumor samples, n = 9 for Grade II, n = 17 for Grade III and n = 7 for Grade IV tumor samples. ( D ) The mean IHC scores for SORT1 is shown for healthy ovarian tissue ( n = 20), benign tumors ( n = 18), LGSC ( n = 21), HGSC ( n = 6), CCC ( n = 5), MC ( n = 3), EC ( n = 13), TCC ( n = 5) and GC and NE ( n = 20). All scatter plots include lines showing the means ± SEM, and where each point represents an individual tissue sample.

Article Snippet: The tissue microarrays (#NBP2-30170 for normal tissues, #NBP2-30290 for ovarian cancers and #NBP2-30330 for diverse cancers) were purchased from Novus Biologicals (Toronto, ON, Canada).

Techniques: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR

Journal: Cancers

Article Title: The Peptide-Drug Conjugate TH1902: A New Sortilin Receptor-Mediated Cancer Therapeutic against Ovarian and Endometrial Cancers

doi: 10.3390/cancers14081877

Figure Lengend Snippet: Expression of SORT1 in endometrial healthy tissues and endometrial tumor biopsies. Immunohistochemical staining of SORT1 in tissue microarrays (TMAs) was used to measure the level of SORT1 expression in healthy tissues and in endometrial tumors. ( A ) Representative SORT1 staining by IHC in endometrial healthy tissue and endometrial tumor biopsies. Nuclei are stained blue with hematoxylin. Black bars represent 50 µm. ( B ) SORT1 IHC scores between healthy endometrial tissues ( n = 2) and endometrial cancerous tissues ( n = 12). Scatter plot include lines showing the means ± SEM, and where each point represents an individual tissue sample.

Article Snippet: The tissue microarrays (#NBP2-30170 for normal tissues, #NBP2-30290 for ovarian cancers and #NBP2-30330 for diverse cancers) were purchased from Novus Biologicals (Toronto, ON, Canada).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Molecular Therapy

Article Title: TALEN-edited allogeneic inducible dual CAR T cells enable effective targeting of solid tumors while mitigating off-tumor toxicity

doi: 10.1016/j.ymthe.2024.08.018

Figure Lengend Snippet: Tumor localized FAP + CAFs inhibit CAR T cell intra-tumoral infiltration and anti-tumor cytotoxicity (A) Schematic illustrating T cell infiltration assay in tumor spheroids plated with or without CAFs. (B) Graph depicting flow cytometry quantitation of T cells infiltrated per tumor spheroids with or without CAFs, represented as percentage of T cell input. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01, ∗∗∗ p ≤ 0.001. (C) Schematic of TRAC KO ML CAR T cell cytotoxicity assay against tumor spheroids of TNBC cell line MDA-MB-231-Luc alone or co-cultured with TNBC-derived CAFs. (D) Bar graph representing percentage MDA-MB-231-Luc tumor cell survival post cytotoxicity assay outlined in (C), at Effector:Target ratio = 5:1. Bars show means ± SD, n = 3; p values determined by Student t test (two-tailed, unpaired). ns, not significant, ∗ p ≤ 0.05, ∗∗ p ≤ 0.01. (E) Immunohistochemistry for detection of human FAP protein in tissue microarray of patient samples from different cancers. (F) Heatmap depicting percentage positive area stained for human FAP protein in immunohistochemical analysis of healthy human tissue microarray.

Article Snippet: Human tumor tissue and healthy tissue microarray slides were obtained from Novus Biologicals (NBP2-30234, NBP2-78082, NBP2-30232, NBP2-30233, NBP2-30189).

Techniques: Flow Cytometry, Quantitation Assay, Two Tailed Test, Cytotoxicity Assay, Cell Culture, Derivative Assay, Immunohistochemistry, Microarray, Staining, Immunohistochemical staining